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CLOSTRIDIUM DIFFICILE LAB-AGAR™ BASE

In stock
Vendor code
PS 176

Selective medium for the isolation of Clostridium difficile from feces.

  • Manufacturer
    BioMaxima
  • Kit format
    1
  • Testing (Manual/Machine/Both)
    Manual and Machine
  • Use by a doctor
    Yes
  • Use by a patient
    No
  • POC
    No
  • Shelf life from the date of production
    70
  • Short Description

    Formula in g/L


    Proteose peptone............................................. 40,00 Fructose. .......................................................... 6,00
    Sodium chloride. ................................................ 2,00 Magnesium sulphate. ...................................... 0,10
    Disodium phosphate .......................................... 5,00 Monopotassium phosphate. ........................... 0,90
    Agar. ................................................................. 15,00


    Final pH at 25°C: 7,4 ± 0,2


    Principle:

    Clostridium difficile LAB-AGAR™ is a selective medium for the isolation of Clostridium difficile. Proteose
    peptone provides nitrogen, vitamins, mineral and amino acids essential for growth. Fructose is the fermentable
    carbohydrate used to enhance recovery and growth of. C. difficile. Disodium phosphate and monopotassium
    phosphate act as a buffer system. Magnesium sulphate ion required in a large variation of enzymatic reactions,
    including DNA replication. Sodium chloride supplies essential electrolytes for transport and osmotic balance.
    Sheep blood provides essential growth factors in Clostridium difficile LAB-AGAR™. The selective agents DCycloserine
    and cefoxitin inhibit the growth of most Enterobacteriaceae, as well as E. faecalis , staphylococci,
    non-sporing anaerobic bacilli and Clostridium spp. except C. difficile), which may be found in large quantities in
    fecal samples.


    Preparation: suspend 34.5 grams of the medium in 500ml of distilled water. Mix well and dissolve by heating
    with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 15
    minutes. Cool to 45-50ºC and aseptically add one vial of Clostridium Difficile Selective Supplement (Cat. no. SL
    0048), previously reconstituted in 4 ml of sterile distilled water and 7% sterile defibrinated horse blood.
    Homogenize gently and dispense into Petri dishes. Be careful to avoid bubble formation when adding the blood
    and rotate the flask or bottle slowly to create a homogeneous solution.
    The prepared medium should be stored at 8-15°C.
    Procedure: